| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2018-04-27 |
| タイトル |
|
|
タイトル |
Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection |
| タイトル |
|
|
タイトル |
Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
sofosbubir |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
hepatitis C virus |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
HPLC |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
UV detection |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
sofosbuvir |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
C 型肝炎ウイルス |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HPLC |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
UV 検出 |
| 著者 |
Su, Nwe San
松本, 准
小池, 雅子
斎藤, 祐実
坂上, 弘明
加藤, 芳徳
有吉, 範高
山田, 治美
|
| 著者別名 |
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|
姓名 |
Su, Nwe San |
| 著者別名 |
|
|
|
姓名 |
Matsumoto, Jun |
| 著者別名 |
|
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|
姓名 |
Koike, Masako |
| 著者別名 |
|
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|
姓名 |
Saito, Yumi |
| 著者別名 |
|
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|
姓名 |
Sakaue, Hiroaki |
| 著者別名 |
|
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|
姓名 |
Kato, Yoshinori |
| 著者別名 |
|
|
|
姓名 |
Ariyoshi, Noritaka |
| 著者別名 |
|
|
|
姓名 |
Yamada, Harumi |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Sofosbuvir (SOF) is a nucleotide analog drug that inhibits hepatitis C virus replication. In the present study, we aimed todevelop a simple, specific, sensitive, and precise method for the quantification of SOF in water-based and human plasmasamples by using a high-performance liquid chromatography method with ultraviolet detection (HPLC-UV). The analyte wasextracted from 0.25 mL water or human plasma samples with an acetonitrile-based solution and analyzed on a C18 reversedphasecolumn. Sorafenib was used as an internal standard (IS). The mobile phase was composed of ammonium acetatebuffer, and methanol and acetonitrile. The flow rate was 1.0 mL/min, and the UV wavelength was set at 265 nm. Theretention times of SOF and IS were 7.8 min and 9.1 min, respectively. The total run time for a single analysis was 15.0 min.The calibration curve was linear within the range 0.1-10.0 μg/mL. The limit of quantitation was 0.1 μg/mL. The recovery ofthe drug from human plasma samples was greater than 99 %. The intra- and inter-day precision of water-based and plasmasamples ranged from 1.41% to 10.06% and 0.21 to 6.53%, respectively. The intra- and inter-day accuracy of water-based andplasma samples ranged from 92.71 to 98.41% and 89.31 to 101.21%, respectively. The proposed method is very simple andallows acquiring a good recovery of the analytes. The developed HPLC-UV method may be applied to conductingpharmacokinetic studies in the determination of SOF. |
| 書誌情報 |
国際医療福祉大学学会誌
en : Journal of the International University of Health and Welfare
巻 23,
号 1,
p. 130-136,
発行日 2018-03-31
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| 出版者 |
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|
出版者 |
国際医療福祉大学学会 |
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
21863652 |
| 書誌レコードID（NCID） |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12537130 |
| 著者版フラグ |
|
|
値 |
publisher |
本文(PDF) (346.8 kB)
