{"created":"2023-05-15T09:17:50.717016+00:00","id":885,"links":{},"metadata":{"_buckets":{"deposit":"f7c83ca5-7920-4403-8676-0e29aa6448a4"},"_deposit":{"created_by":15,"id":"885","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"885"},"status":"published"},"_oai":{"id":"oai:iuhw.repo.nii.ac.jp:00000885","sets":["2:137"]},"author_link":["2000","1655","2001","1999","2004","2006","358","1998","2007","361","2008","370","2003","2002","367","2005"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"136","bibliographicPageStart":"130","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"国際医療福祉大学学会誌"},{"bibliographic_title":"Journal of the International University of Health and Welfare","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" Sofosbuvir (SOF) is a nucleotide analog drug that inhibits hepatitis C virus replication. In the present study, we aimed todevelop a simple, specific, sensitive, and precise method for the quantification of SOF in water-based and human plasmasamples by using a high-performance liquid chromatography method with ultraviolet detection (HPLC-UV). The analyte wasextracted from 0.25 mL water or human plasma samples with an acetonitrile-based solution and analyzed on a C18 reversedphasecolumn. Sorafenib was used as an internal standard (IS). The mobile phase was composed of ammonium acetatebuffer, and methanol and acetonitrile. The flow rate was 1.0 mL/min, and the UV wavelength was set at 265 nm. Theretention times of SOF and IS were 7.8 min and 9.1 min, respectively. The total run time for a single analysis was 15.0 min.The calibration curve was linear within the range 0.1-10.0 μg/mL. The limit of quantitation was 0.1 μg/mL. The recovery ofthe drug from human plasma samples was greater than 99 %. The intra- and inter-day precision of water-based and plasmasamples ranged from 1.41% to 10.06% and 0.21 to 6.53%, respectively. The intra- and inter-day accuracy of water-based andplasma samples ranged from 92.71 to 98.41% and 89.31 to 101.21%, respectively. The proposed method is very simple andallows acquiring a good recovery of the analytes. The developed HPLC-UV method may be applied to conductingpharmacokinetic studies in the determination of SOF.","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":" Sofosbuvir (SOF) is a nucleotide analog drug that inhibits hepatitis C virus replication. In the present study, we aimed todevelop a simple, specific, sensitive, and precise method for the quantification of SOF in water-based and human plasmasamples by using a high-performance liquid chromatography method with ultraviolet detection (HPLC-UV). The analyte wasextracted from 0.25 mL water or human plasma samples with an acetonitrile-based solution and analyzed on a C18 reversedphasecolumn. Sorafenib was used as an internal standard (IS). The mobile phase was composed of ammonium acetatebuffer, and methanol and acetonitrile. The flow rate was 1.0 mL/min, and the UV wavelength was set at 265 nm. Theretention times of SOF and IS were 7.8 min and 9.1 min, respectively. The total run time for a single analysis was 15.0 min.The calibration curve was linear within the range 0.1-10.0 μg/mL. The limit of quantitation was 0.1 μg/mL. The recovery ofthe drug from human plasma samples was greater than 99 %. The intra- and inter-day precision of water-based and plasmasamples ranged from 1.41% to 10.06% and 0.21 to 6.53%, respectively. The intra- and inter-day accuracy of water-based andplasma samples ranged from 92.71 to 98.41% and 89.31 to 101.21%, respectively. The proposed method is very simple andallows acquiring a good recovery of the analytes. The developed HPLC-UV method may be applied to conductingpharmacokinetic studies in the determination of SOF.","subitem_description_type":"Other"}]},"item_10001_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"Su, Nwe San"}]},{"nameIdentifiers":[{}],"names":[{"name":"Matsumoto, Jun"}]},{"nameIdentifiers":[{}],"names":[{"name":"Koike, Masako"}]},{"nameIdentifiers":[{}],"names":[{"name":"Saito, Yumi"}]},{"nameIdentifiers":[{}],"names":[{"name":"Sakaue, Hiroaki"}]},{"nameIdentifiers":[{}],"names":[{"name":"Kato, Yoshinori"}]},{"nameIdentifiers":[{}],"names":[{"name":"Ariyoshi, Noritaka"}]},{"nameIdentifiers":[{}],"names":[{"name":"Yamada, Harumi"}]}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国際医療福祉大学学会"}]},"item_10001_radio_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_radio_item":"publisher"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID（NCID）","attribute_value_mlt":[{"subitem_source_identifier":"AA12537130","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21863652","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Su, Nwe San"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本, 准"},{"creatorName":"マツモト, ジュン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"小池, 雅子"},{"creatorName":"コイケ, マサコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"斎藤, 祐実"},{"creatorName":"サイトウ, ユミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"坂上, 弘明"},{"creatorName":"サカウエ, ヒロアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤, 芳徳"},{"creatorName":"カトウ, ヨシノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"有吉, 範高"},{"creatorName":"アリヨシ, ノリタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山田, 治美"},{"creatorName":"ヤマダ, ハルミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-27"}],"displaytype":"simple","filename":"23_1(130-136p).pdf","filesize":[{"value":"346.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文(PDF)","url":"https://iuhw.repo.nii.ac.jp/record/885/files/23_1(130-136p).pdf"},"version_id":"396bfb8f-6e02-4c53-ac2c-75f794345a5b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"sofosbubir","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatitis C virus","subitem_subject_scheme":"Other"},{"subitem_subject":"HPLC","subitem_subject_scheme":"Other"},{"subitem_subject":"UV detection","subitem_subject_scheme":"Other"},{"subitem_subject":"sofosbuvir","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"C 型肝炎ウイルス","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HPLC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"UV 検出","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection"},{"subitem_title":"Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["137"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-27"},"publish_date":"2018-04-27","publish_status":"0","recid":"885","relation_version_is_last":true,"title":["Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-21T10:40:58.191372+00:00"}