@article{oai:iuhw.repo.nii.ac.jp:00000885,
 author = {Su, Nwe San and 松本, 准 and 小池, 雅子 and 斎藤, 祐実 and 坂上, 弘明 and 加藤, 芳徳 and 有吉, 範高 and 山田, 治美},
 issue = {1},
 journal = {国際医療福祉大学学会誌, Journal of the International University of Health and Welfare},
 month = {Mar},
 note = {Sofosbuvir (SOF) is a nucleotide analog drug that inhibits hepatitis C virus replication. In the present study, we aimed todevelop a simple, specific, sensitive, and precise method for the quantification of SOF in water-based and human plasmasamples by using a high-performance liquid chromatography method with ultraviolet detection (HPLC-UV). The analyte wasextracted from 0.25 mL water or human plasma samples with an acetonitrile-based solution and analyzed on a C18 reversedphasecolumn. Sorafenib was used as an internal standard (IS). The mobile phase was composed of ammonium acetatebuffer, and methanol and acetonitrile. The flow rate was 1.0 mL/min, and the UV wavelength was set at 265 nm. Theretention times of SOF and IS were 7.8 min and 9.1 min, respectively. The total run time for a single analysis was 15.0 min.The calibration curve was linear within the range 0.1-10.0 μg/mL. The limit of quantitation was 0.1 μg/mL. The recovery ofthe drug from human plasma samples was greater than 99 %. The intra- and inter-day precision of water-based and plasmasamples ranged from 1.41% to 10.06% and 0.21 to 6.53%, respectively. The intra- and inter-day accuracy of water-based andplasma samples ranged from 92.71 to 98.41% and 89.31 to 101.21%, respectively. The proposed method is very simple andallows acquiring a good recovery of the analytes. The developed HPLC-UV method may be applied to conductingpharmacokinetic studies in the determination of SOF.},
 pages = {130--136},
 title = {Determination of sofosbuvir via a high-performance liquid chromatography method using ultraviolet detection},
 volume = {23},
 year = {2018},
 yomi = {マツモト, ジュン and コイケ, マサコ and サイトウ, ユミ and サカウエ, ヒロアキ and カトウ, ヨシノリ and アリヨシ, ノリタカ and ヤマダ, ハルミ}
}